Microplate readers find and process biological & chemical data by using absorbance, luminescence, and fluorescence detection modes. ELISA is very common and standard and pre-coated plates can be purchased. These prepared plates can be used for clinical or experimental samples after opening.
ELISA Microplate Reader has 8-channel vertical optical system. Its reading speed is about 7 seconds. ELISA means enzyme linked immunosorbent assay that is an evaluation technique for immobilizing an antigen to a solid surface to determine peptide, protein, antibody, or hormone concentrations. Immunoassay uses special lock and key detection between antibodies and antigens.
ELIZA plate reader uses an automated microplate reader with a number of microplates that act as tiny test tubes combined in a single molded plastic plate. The microplate reader is a necessary tool in the field of biological research and observation such as cancer detection and pharmaceutical discovery, development and Q.C.
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What Is the ELISA Reader
Biochemists test fluid samples to see if hormones, substances, antibodies proteins, or other antigens are present in the solution. ELISA reader is a specialized spectrophotometer to measure light intensity and allow biochemists to measure the reactions of antigens and antibodies in a solution through enzyme activity. The ELISA is performed in a several well polystyrene plate in one of two formats: either antigen is attached to the bottom of each well, or the capture antibody is attached to the bottom of each well.
A typical microplate contains 8 columns and 12 rows for 96 sample wells. It allows you to check up to 96 different conditions simultaneously. The automated ELISA reader has an optical system that analyzes samples with light so that users can interpret the results.
Indirect ELISA
Indirect ELISA works with a two-step process. First, a primary antibody is added to the sample. A secondary antibody is then added to bind to the primary antibody. This method has high flexibility and signal amplification. However, it may result in cross-reaction from the secondary antibody, making the result inconclusive.
Direct ELISA
During the direct ELISA test, the antigen is added directly to the microplate well. The antibody is then added to the solution and binds directly to the antigen to prepare it for the addition of another enzyme. Finally, the next test enzyme is added and analyzed by the color change of the test. Advantages of this test include fewer steps, fewer reagents needed, and no secondary antibody reactions that can confound the results.
The ELISA is such a common and standardized assay that it is possible to purchase pre-coated plates that are pre-blocked and ready to receive the clinical or experimental sample immediately upon opening the package.
Application of Eliza Reader
ELISA readers have many uses. ELISA can detect the following diseases: AIDS, Ebola, rotavirus, Lyme disease, anemia, cancer, and rotavirus. During the COVID-19 outbreak, ELISA testers helped detect the presence of antibodies in blood samples. Also, ELISA reader can be used in the food industry to detect any existing food allergens.
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