BioLegend products are about world-class advanced antibodies and reagents for biotechnology research. These products are manufactured at BioLegend factory in San Diego, California. BioLegend products accelerate biotechnology research and discovery by providing the highest quality products at exceptional prices, along with superior customer service and technical support.
BioLegend application
BioLegend’s product property spans a wide range of research areas, including neuroscience, immunology, stem cells, cancer and cell biology. BioLegend’s reagents are backed by superior customer service and an ISO 13485:2016 certified quality management system. Aggressive product development program through in-house hybridoma development, collaboration and technology licensing provides strategic reagents for use in a variety of applications, including:
- Quantitative multiplexing
- Flow cytometry and mass cytometry
- Immunofluorescence microscopy
- Immunohistochemistry
- ELISA and ELISPOT
- Western blot
- Functional testing in vitro or in vivo
- Safety deposit and ChIP
biolegend flow cytometry
BioLegend products offer a broad selection of fluorochrome conjugates for multicolor flow cytometry. BioLegend have an experienced team in biochemistry and well positioned to offer a wide range of conjugation services tailored to meet the specific needs of customers.
Research area
Research areas of BioLegend products including:
- Immunology
- Proteogenomics
- Oncology
- Clinical Flow Cytometry
- Stem Cells
- Neuroscience
BioLegend products Type
The types of BioLegend products are very wide, which we will describe below in a headline-wise manner:
- Non-Human primate antibodies
- Buffers and solutions
- Recombinant proteins, Cytokines, Growth Factors
- Isotype Controls
- Magnetic cell separation – MojoSort™
- Soluble MHC ‐ Flex‐T™
- Ultra-LEAF™ | GoInVivo™ Antibodies
- Apoptosis and cell health reagents
- Clinical and analyte specific reagents
- GMP reagents
- Secondary reagents
- SARS-CoV-2 Reagents
- Primary antibodies
types of antibodies
In the following, different types of antibodies have been introduced in BioLegend products:
primary antibody
BioLegend provided quality-tested, validated, highly specific primary antibodies by Western Blot for the detection of proteins of interest. BioLegend antibodies cover a wide range of targets in neuroscience, cell biology, immunology and other important signaling pathways. They come in a pure format, unconjugated or directly conjugated with HRP.
secondary reagents
Secondary reagents are directed to primary antibodies and conjugated to allow visualization of the protein of interest. For western blot, these reagents are conjugated with HRP used as reporter enzyme.
knockout/knockdown verification
BioLegend validated many cell biology antibodies with KO (knockout) and KD (knockdown) systems, as removal of target proteins is one of the most reliable antibody validation procedures.
Reproducibility of published research has become an important issue in today’s scientific community. It is critical that all these groups, from donors and researchers to producers and publishers, align to ensure
that research is conducted with rigor and reproducibility.
Phosphatic Antibody Kit
Phosphatic Antibody Kit for a convenient, cost-effective solution for interrogating cellular signaling and phosphorylation pathways have been produced. Each set contains an antibody specific for a phosphorylated protein and an antibody that recognizes the total protein. Kits are available for important signaling mediators such as STAT3, ERK, and mTOR. For more information about BioLegend products, follow the contact page.
key features:
• It is supplied in a purified format at 25 mcg per vial.
• The quality of each antibody is tested by western blot.
Antibody Sample Kit
Antibody sample kits provide a convenient and inexpensive resource for testing multiple antibodies. Each kit of BioLegend products contains at least 4 antibody and protein kits with target cell function and structure, associated detection methods such as epitope tags, or proteins that bind through signaling pathways.
protein balance
The Prime-Step Pre-Stained Broad-Range Protein Ladder is a tricolor protein standard consisting of 10 recombinant proteins conjugated to a pre-Stained chromophore, covering a broad molecular weight range from 6.5 to 270 kDa. These BioLegend products are ideal for monitoring protein dissociation during SDS-PAGE, confirming WB transfer to membranes, and estimating protein size.
ECL surfaces
BioLegend Western-Ready ECL Substrate products are non-radioactive luminol-based chemiluminescent (ECL) substrates for the detection of horseradish peroxidase (HRP) activity from WB reagents and ELISA immunoassays. Our kits provide accurate detection and continuous signaling for up to 4 hours. Our ECL Plus kit is ideal for the detection of low to moderate antigen levels in picograms, while the Premium kit provides more sensitive detection of high femtogram protein levels.
Main characteristics:
• accurate identification
• Luminol based detection system
• Multiple kits with a range of sensitivities
• Available in a smaller 20ml size for samples
Western-Ready Pad
BioLegend complete line of Western-Ready buffers products is designed for every step of the workflow, including protein extraction, gel loading and running, and detection.
| types | usage |
| Western-Ready MES SDS-PAGE Running Buffer (10X) | Buffer that serves to resolve small to medium sized proteins on Bis-Tris SDS PAGE gels. |
| Western-Ready Protein Sample Loading Buffer (5X) | It is used in sample preparation for non-reducing and depleting SDS-PAGE. |
| Western-Ready Rapid Protein Extraction Buffer | Solid Lysis Buffer to extract proteins from all subcellular compartments. |
| Western-Ready TBS Tween-20 Buffer (20X) | Antibody blocking, washing and dilution buffer. Formulated to reduce non-specific binding. |
| Western-Ready Transfer Buffer (10X) | Prepare buffer for western blot protein transfer to PVDF or nitrocellulose membranes. |
Last point
In this article, we tried to give comprehensive and useful information about BioLegend products. If you have any questions about this, you can ask through the box below so that you will be answered as soon as possible. Vesta, as one of the most important suppliers of laboratory equipment in Iran, is trying to supply the best equipment for the medical field. Contact Vesta for more information.
